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No.
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Size
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Price
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M20008S
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50 μl
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￥500
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M20008M
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100 μl
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￥900
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M20008L
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200 μl
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￥1600
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Product Name
DYKDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma’s Anti-FLAG® M2 Antibody)
Source
This Abmart monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).
Specifity
DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.
Storage
Store at -20°C. Stable for one year from the date of shipment.
Secondary Antibody (Important)
Use an anti-MOUSE secondary antibody to detect the 3B9 antibody.
Application Tested
Western blotting 1:5000Immunofluorescence 1:1000Immunoprecipitation 1:100ELISA 1:2000
Application Data
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Western blot analysis of over-expressed DYKDDDDK-tagged protein in 293T cell lysate, using Abmart DYKDDDDK-Tag (3B9) Mouse mAb. The antibody dilutions are 1:2000 (lane 1), 1:5000 (lane 2), 1:10000 (lane 3) and 1:20000 (lane 4). Each lane was loaded with 2 μg of cell lysate. Lane 5 is 2 μg of mock transfected cell lysate at a dilution of 1:5000.
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IF analysis of 293T cells transfected with a DYKDDDDK-tagged protein, using Abmart DYKDDDDK-Tag (3B9) Mouse mAb at a 1:1000 dilution.
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Lane 1: 293T cell lysate transfected with DYKDDDDK-tag protein.Lane 2: Mock transfected 293T cell lysate.The antibody dilution is 1:100.
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AbMATE-2A8 A positive control for using
3B9
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Failure to see above result suggests that your Western experiment system is NOT
working, and likely your own Western experiment will not work with
3B9. If you see the expected band and the dot (3B9
is working), but your own experiment membrane is not what you expected, please look
for other reasons (for example, your protein sample preparation) rather than Western
system itself.
Please contact Abmart’s technical support team by phone (+86-21-64048322) or email
(support@abmart.com.cn) for further questions.
AbMATE-3B9
can be stored at 4°C for 6 months.
(The AbMATE-3B9
should be submerged in methanol for 5 minutes before using).
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Membrane before experiment
Film after experiment
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Companion Products
For in vitro research use only and not intended for use in humans or animals